Dissecting elastic heterogeneity along DNA molecules coated partly with Rad51 using concurrent fluorescence microscopy and optical tweezers.

Combining optical tweezers and scanning probe microscopy to study DNA-protein interactions.

We present the first results obtained with a new instrument designed and built to study DNA-protein interactions at the single molecule level. This microscope combines optical tweezers with scanning probe microscopy and allows us to locate DNA-binding proteins on a single suspended DNA molecule. A single DNA molecule is stretched taut using the optical tweezers, while a probe is scanned along t...

Developing a new biophysical tool to combine magneto-optical tweezers with super-resolution fluorescence microscopy

We present a novel experimental setup in which magnetic and optical tweezers are combined for torque and force transduction onto single filamentous molecules in a transverse configuration to allow simultaneous mechanical measurement and manipulation. Previously we have developed a super-resolution imaging module which in conjunction with advanced imaging techniques such as Blinking assisted Loc...

Single-Molecule Studies of DNA Visualization and Manipulation of Individual DNA Molecules with Fluorescence Microscopy and Optical Tweezers

Magnetic Tweezers for Single-Molecule Experiments

Over the last decade, single-molecule techniques have proven their wide applicability in the study of processive motor proteins and other enzymes, yielding insight into their kinetics and mechanochemistry. In the context of force spectroscopy of protein–nucleic acid interactions, optical tweezers, magnetic tweezers, and atomic force microscopy have made important contributions. Advantages of ma...

Combining optical trapping, fluorescence microscopy and micro-fluidics for single molecule studies of DNA-protein interactions.

Complexity and heterogeneity are common denominators of the many molecular events taking place inside the cell. Single-molecule techniques are important tools to quantify the actions of biomolecules. Heterogeneous interactions between multiple proteins, however, are difficult to study with these technologies. One solution is to integrate optical trapping with micro-fluidics and single-molecule ...